Inducible expression of antigens in plants: a study focused on peptides related to multiple sclerosis immunotherapy.
Identifieur interne : 000131 ( Main/Exploration ); précédent : 000130; suivant : 000132Inducible expression of antigens in plants: a study focused on peptides related to multiple sclerosis immunotherapy.
Auteurs : Jaime I. Arevalo-Villalobos [Mexique] ; Dania O. Govea-Alonso [Mexique] ; Bernardo Ba Uelos-Hernández [Mexique] ; Omar González-Ortega [Mexique] ; Sergio Zarazúa [Mexique] ; Sergio Rosales-Mendoza [Mexique]Source :
- Journal of biotechnology [ 1873-4863 ] ; 2020.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Cysteine endopeptidases (génétique), Expression des gènes (MeSH), Fragments peptidiques (génétique), Fragments peptidiques (immunologie), Humains (MeSH), Immunisation (MeSH), Immunothérapie (MeSH), Protéines virales (génétique), Récepteurs aux antigènes des cellules T (génétique), Récepteurs aux antigènes des cellules T (immunologie), Régions promotrices (génétique) (génétique), Sclérose en plaques (immunologie), Sclérose en plaques (thérapie), Souris (MeSH), Tabac (génétique), Tabac (métabolisme), Vaccins sous-unitaires (génétique), Vaccins sous-unitaires (immunologie), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- génétique : Cysteine endopeptidases, Fragments peptidiques, Protéines virales, Récepteurs aux antigènes des cellules T, Régions promotrices (génétique), Tabac, Vaccins sous-unitaires, Végétaux génétiquement modifiés.
- immunologie : Fragments peptidiques, Récepteurs aux antigènes des cellules T, Sclérose en plaques, Vaccins sous-unitaires.
- métabolisme : Tabac, Végétaux génétiquement modifiés.
- thérapie : Sclérose en plaques.
- Animaux, Expression des gènes, Humains, Immunisation, Immunothérapie, Souris, Vecteurs génétiques.
English descriptors
- KwdEn :
- Animals (MeSH), Cysteine Endopeptidases (genetics), Gene Expression (MeSH), Genetic Vectors (MeSH), Humans (MeSH), Immunization (MeSH), Immunotherapy (MeSH), Mice (MeSH), Multiple Sclerosis (immunology), Multiple Sclerosis (therapy), Peptide Fragments (genetics), Peptide Fragments (immunology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Promoter Regions, Genetic (genetics), Receptors, Antigen, T-Cell (genetics), Receptors, Antigen, T-Cell (immunology), Tobacco (genetics), Tobacco (metabolism), Vaccines, Subunit (genetics), Vaccines, Subunit (immunology), Viral Proteins (genetics).
- MESH :
- chemical , genetics : Cysteine Endopeptidases, Peptide Fragments, Receptors, Antigen, T-Cell, Vaccines, Subunit, Viral Proteins.
- genetics : Plants, Genetically Modified, Promoter Regions, Genetic, Tobacco.
- immunology : Multiple Sclerosis, Peptide Fragments, Receptors, Antigen, T-Cell, Vaccines, Subunit.
- metabolism : Plants, Genetically Modified, Tobacco.
- therapy : Multiple Sclerosis.
- Animals, Gene Expression, Genetic Vectors, Humans, Immunization, Immunotherapy, Mice.
Abstract
Multiple sclerosis (MS) affects 2.3 million patients worldwide with no effective treatments available thus far. Depletion of autoreactive T-cells is considered the basis for immunotherapeutic approaches. For this purpose the peptides BV5S2, BV6S5, and BV13S1 have been identified as candidates for the development of a MS vaccine. Herein, the plant-based simultaneous production of these peptides is described as an effort to generate a new model of MS immunotherapy. A polyprotein comprising the sequence of the target peptides was designed having the picornaviral 2A sequence in between to mediate the release of the individual peptides upon translation. A codon optimized gene was cloned in vectors mediating constitutive (CaMV35S promoter) or inducible (AlcA promoter) expression. No transgenic tobacco plants were recovered from the constitutive vector suggesting toxicity of the target peptides. In contrast, several transformed lines were obtained with the inducible vector. The individual BV5S2, BV6S5, and BV13S1 peptides were detected in transformed lines upon ethanol-mediated induction and a quantitative analysis based on a OVA conjugate carrying the three peptides revealed accumulation levels up to 0.5 μg g-1 FW leaves. The plant-made peptides were able to induce humoral responses in orally immunized mice. This platform will be useful in the development of alternative immunotherapies against MS having low cost and safety as main attributes. Moreover the platform represents an attractive alternative for the expression of antigens having detrimental effects in plants.
DOI: 10.1016/j.jbiotec.2020.03.013
PubMed: 32387449
Affiliations:
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Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. 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Arevalo-Villalobos</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210</wicri:regionArea><wicri:noRegion>78210</wicri:noRegion></affiliation></author><author><name sortKey="Govea Alonso, Dania O" sort="Govea Alonso, Dania O" uniqKey="Govea Alonso D" first="Dania O" last="Govea-Alonso">Dania O. Govea-Alonso</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210</wicri:regionArea><wicri:noRegion>78210</wicri:noRegion></affiliation></author><author><name sortKey="Ba Uelos Hernandez, Bernardo" sort="Ba Uelos Hernandez, Bernardo" uniqKey="Ba Uelos Hernandez B" first="Bernardo" last="Ba Uelos-Hernández">Bernardo Ba Uelos-Hernández</name><affiliation wicri:level="1"><nlm:affiliation>Escuela de Veterinaria, Universidad De La Salle Bajío, Avenida Universidad 602, Lomas del Campestre, 37150, León, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Escuela de Veterinaria, Universidad De La Salle Bajío, Avenida Universidad 602, Lomas del Campestre, 37150, León, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez Ortega, Omar" sort="Gonzalez Ortega, Omar" uniqKey="Gonzalez Ortega O" first="Omar" last="González-Ortega">Omar González-Ortega</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210</wicri:regionArea><wicri:noRegion>78210</wicri:noRegion></affiliation></author><author><name sortKey="Zarazua, Sergio" sort="Zarazua, Sergio" uniqKey="Zarazua S" first="Sergio" last="Zarazúa">Sergio Zarazúa</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210</wicri:regionArea><wicri:noRegion>78210</wicri:noRegion></affiliation></author><author><name sortKey="Rosales Mendoza, Sergio" sort="Rosales Mendoza, Sergio" uniqKey="Rosales Mendoza S" first="Sergio" last="Rosales-Mendoza">Sergio Rosales-Mendoza</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico. Electronic address: rosales.s@uaslp.mx.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210</wicri:regionArea><wicri:noRegion>78210</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="eISSN">1873-4863</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Cysteine Endopeptidases (genetics)</term><term>Gene Expression (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Humans (MeSH)</term><term>Immunization (MeSH)</term><term>Immunotherapy (MeSH)</term><term>Mice (MeSH)</term><term>Multiple Sclerosis (immunology)</term><term>Multiple Sclerosis (therapy)</term><term>Peptide Fragments (genetics)</term><term>Peptide Fragments (immunology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>Receptors, Antigen, T-Cell (genetics)</term><term>Receptors, Antigen, T-Cell (immunology)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Vaccines, Subunit (genetics)</term><term>Vaccines, Subunit (immunology)</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Cysteine endopeptidases (génétique)</term><term>Expression des gènes (MeSH)</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (immunologie)</term><term>Humains (MeSH)</term><term>Immunisation (MeSH)</term><term>Immunothérapie (MeSH)</term><term>Protéines virales (génétique)</term><term>Récepteurs aux antigènes des cellules T (génétique)</term><term>Récepteurs aux antigènes des cellules T (immunologie)</term><term>Régions promotrices (génétique) (génétique)</term><term>Sclérose en plaques (immunologie)</term><term>Sclérose en plaques (thérapie)</term><term>Souris (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Vaccins sous-unitaires (génétique)</term><term>Vaccins sous-unitaires (immunologie)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cysteine Endopeptidases</term><term>Peptide Fragments</term><term>Receptors, Antigen, T-Cell</term><term>Vaccines, Subunit</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cysteine endopeptidases</term><term>Fragments peptidiques</term><term>Protéines virales</term><term>Récepteurs aux antigènes des cellules T</term><term>Régions promotrices (génétique)</term><term>Tabac</term><term>Vaccins sous-unitaires</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Fragments peptidiques</term><term>Récepteurs aux antigènes des cellules T</term><term>Sclérose en plaques</term><term>Vaccins sous-unitaires</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Multiple Sclerosis</term><term>Peptide Fragments</term><term>Receptors, Antigen, T-Cell</term><term>Vaccines, Subunit</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Multiple Sclerosis</term></keywords><keywords scheme="MESH" qualifier="thérapie" xml:lang="fr"><term>Sclérose en plaques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Gene Expression</term><term>Genetic Vectors</term><term>Humans</term><term>Immunization</term><term>Immunotherapy</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Humains</term><term>Immunisation</term><term>Immunothérapie</term><term>Souris</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Multiple sclerosis (MS) affects 2.3 million patients worldwide with no effective treatments available thus far. Depletion of autoreactive T-cells is considered the basis for immunotherapeutic approaches. For this purpose the peptides BV5S2, BV6S5, and BV13S1 have been identified as candidates for the development of a MS vaccine. Herein, the plant-based simultaneous production of these peptides is described as an effort to generate a new model of MS immunotherapy. A polyprotein comprising the sequence of the target peptides was designed having the picornaviral 2A sequence in between to mediate the release of the individual peptides upon translation. A codon optimized gene was cloned in vectors mediating constitutive (CaMV35S promoter) or inducible (AlcA promoter) expression. No transgenic tobacco plants were recovered from the constitutive vector suggesting toxicity of the target peptides. In contrast, several transformed lines were obtained with the inducible vector. The individual BV5S2, BV6S5, and BV13S1 peptides were detected in transformed lines upon ethanol-mediated induction and a quantitative analysis based on a OVA conjugate carrying the three peptides revealed accumulation levels up to 0.5 μg g<sup>-1</sup> FW leaves. The plant-made peptides were able to induce humoral responses in orally immunized mice. This platform will be useful in the development of alternative immunotherapies against MS having low cost and safety as main attributes. Moreover the platform represents an attractive alternative for the expression of antigens having detrimental effects in plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32387449</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4863</ISSN><JournalIssue CitedMedium="Internet"><Volume>318</Volume><PubDate><Year>2020</Year><Month>Jul</Month><Day>20</Day></PubDate></JournalIssue><Title>Journal of biotechnology</Title><ISOAbbreviation>J Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Inducible expression of antigens in plants: a study focused on peptides related to multiple sclerosis immunotherapy.</ArticleTitle><Pagination><MedlinePgn>51-56</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0168-1656(20)30076-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiotec.2020.03.013</ELocationID><Abstract><AbstractText>Multiple sclerosis (MS) affects 2.3 million patients worldwide with no effective treatments available thus far. Depletion of autoreactive T-cells is considered the basis for immunotherapeutic approaches. For this purpose the peptides BV5S2, BV6S5, and BV13S1 have been identified as candidates for the development of a MS vaccine. Herein, the plant-based simultaneous production of these peptides is described as an effort to generate a new model of MS immunotherapy. A polyprotein comprising the sequence of the target peptides was designed having the picornaviral 2A sequence in between to mediate the release of the individual peptides upon translation. A codon optimized gene was cloned in vectors mediating constitutive (CaMV35S promoter) or inducible (AlcA promoter) expression. No transgenic tobacco plants were recovered from the constitutive vector suggesting toxicity of the target peptides. In contrast, several transformed lines were obtained with the inducible vector. The individual BV5S2, BV6S5, and BV13S1 peptides were detected in transformed lines upon ethanol-mediated induction and a quantitative analysis based on a OVA conjugate carrying the three peptides revealed accumulation levels up to 0.5 μg g<sup>-1</sup> FW leaves. The plant-made peptides were able to induce humoral responses in orally immunized mice. This platform will be useful in the development of alternative immunotherapies against MS having low cost and safety as main attributes. Moreover the platform represents an attractive alternative for the expression of antigens having detrimental effects in plants.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arevalo-Villalobos</LastName><ForeName>Jaime I</ForeName><Initials>JI</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govea-Alonso</LastName><ForeName>Dania O</ForeName><Initials>DO</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bañuelos-Hernández</LastName><ForeName>Bernardo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Escuela de Veterinaria, Universidad De La Salle Bajío, Avenida Universidad 602, Lomas del Campestre, 37150, León, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>González-Ortega</LastName><ForeName>Omar</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zarazúa</LastName><ForeName>Sergio</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosales-Mendoza</LastName><ForeName>Sergio</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2ª Sección, San Luis Potosí, 78210, Mexico. Electronic address: rosales.s@uaslp.mx.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>05</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biotechnol</MedlineTA><NlmUniqueID>8411927</NlmUniqueID><ISSNLinking>0168-1656</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011948">Receptors, Antigen, T-Cell</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D022223">Vaccines, Subunit</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D003546">Cysteine Endopeptidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.29</RegistryNumber><NameOfSubstance UI="C057400">picornain 2A, Picornavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003546" MajorTopicYN="N">Cysteine Endopeptidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007114" MajorTopicYN="N">Immunization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007167" MajorTopicYN="Y">Immunotherapy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009103" MajorTopicYN="N">Multiple Sclerosis</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000628" MajorTopicYN="Y">therapy</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011948" MajorTopicYN="N">Receptors, Antigen, T-Cell</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D022223" MajorTopicYN="N">Vaccines, Subunit</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">2A peptide</Keyword><Keyword MajorTopicYN="N">Algevir</Keyword><Keyword MajorTopicYN="N">T cell receptor peptide</Keyword><Keyword MajorTopicYN="N">multicomponent vaccine</Keyword><Keyword MajorTopicYN="N">multiple sclerosis immunotherapy</Keyword><Keyword MajorTopicYN="N">plant-made vaccine</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare no conflict of interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32387449</ArticleId><ArticleId IdType="pii">S0168-1656(20)30076-6</ArticleId><ArticleId IdType="doi">10.1016/j.jbiotec.2020.03.013</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Mexique</li></country></list><tree><country name="Mexique"><noRegion><name sortKey="Arevalo Villalobos, Jaime I" sort="Arevalo Villalobos, Jaime I" uniqKey="Arevalo Villalobos J" first="Jaime I" last="Arevalo-Villalobos">Jaime I. Arevalo-Villalobos</name></noRegion><name sortKey="Ba Uelos Hernandez, Bernardo" sort="Ba Uelos Hernandez, Bernardo" uniqKey="Ba Uelos Hernandez B" first="Bernardo" last="Ba Uelos-Hernández">Bernardo Ba Uelos-Hernández</name><name sortKey="Gonzalez Ortega, Omar" sort="Gonzalez Ortega, Omar" uniqKey="Gonzalez Ortega O" first="Omar" last="González-Ortega">Omar González-Ortega</name><name sortKey="Govea Alonso, Dania O" sort="Govea Alonso, Dania O" uniqKey="Govea Alonso D" first="Dania O" last="Govea-Alonso">Dania O. Govea-Alonso</name><name sortKey="Rosales Mendoza, Sergio" sort="Rosales Mendoza, Sergio" uniqKey="Rosales Mendoza S" first="Sergio" last="Rosales-Mendoza">Sergio Rosales-Mendoza</name><name sortKey="Zarazua, Sergio" sort="Zarazua, Sergio" uniqKey="Zarazua S" first="Sergio" last="Zarazúa">Sergio Zarazúa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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